2008•Acta Academiae Medicinae JiangxiRequires access
Expression of Transplantation of Microencapsulated Rabbit Sciatic Nerve on the Expression of IκBα after Spinal Cord Injury in Rats
Liu De-mingb
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Abstract
Objective To investigate the effects of transplantation of microencapsulated rabbit sciatic nerve tissue/cells on IκBα activity and expression after spinal cord injury(SCI) in rats.Methods The rabbit sciatic nerve tissue/cells suspension was prepared from ten rabbits.SCI was conducted by hemisection injury of spinal cord.A total of 120 adult Sprague Dawley rats were randomly divided into four groups:36 rats in microencapsulated group(10 μL microencapsulated sciatic nerve tissue/cells suspension absorbed in gelatin sponge was transplanted into the SCI site immediately after SCI),36 rats in cellular group(10 μL sciatic nerve tissue/cells suspension absorbed in gelatin sponge was transplanted into the SCI site immediately after SCI),36 rats in injury control group(10 μL physiological saline absorbed in gelatin sponge was administered to the SCI site immediately after SCI),12 rats in sham-injury controll group(spinal cord was neither injured nor transplanted,with vertebrae removed only).For microencapsulated group,cellular group,and injury control group,spinal cord samples were taken out from 6 rats in each group respectively 6 h,12 h,24 h,3 d,7 d,and 14 d after SCI,while corresponding samples were collected from 2 rats in sham-injury group each time.All samples were postfixed and embedded in paraffin and then sectioned,followed by undergoing immunohistochemical stain for detection of IκBα.ResultsThe IκBα protein are mainly seen in the cytoplasm of neurons and gliocytes.The expression of IκBα decreased and reached the lowest leve1 24 hours after SCI,and began to rise 3 d after,and gradually returned to normal level 7 d after.The expression level of IκBαin microcapsulated group was significantly different from those in injury control group and cellular group(P0.05).ConclusionIt suggests that the transplantation of microencapsulated rabbit sciatic nerve tissue/cells could inhibit the phosphorylation of IκBα,thus accordingly suppress inflammatory reaction after spinal cord injury in rats.