2004•Xibei zhiwu xuebaoRequires access
An optimum method of extracting plant genomic DNA
XU Ziqin
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Abstract
The CTAB method of extracting plant genomic DNA of Clark's was improved according to the experiences of ourselves. Plant materials were ground in liquid nitrogen. DNA solution was transferred with pipettetip without top. The digestion step of RNase A was carried out at the last step. High-quality DNA could be obtained on a series of these changes. In addition, major procedures of the CTAB method of extracting plant DNA of Clark's were discussed.