2009•Research and Exploration in LaboratoryRequires access
CTAB-improved Method of DNA Extraction in Plant
Ronghua Li
Open publisher page 9 citations
Abstract
On basis of the physical and chemical characteristics of DNA molecule and the traits of plant tissues,an improvement of DNA isolation method with CTAB was made.The progresses in the improved method mainly include: ① to use fresh CTAB solution,② to grind plant tissues with liquid nitrogen,③ to add suitable reducant in extraction solution and ④ to pipette appropriate nucleic acid solution from supernatant.These measures can prevent brown stain in extraction solution and reduce impurities.In addition,notes and key points for the procedures of DNA isolation were described in the method.Its operation is simple and rapid,the DNA isolated with this method can be efficiently used for the analysis of DNA molecular genetic characteristics.Therefore,it is a suitable method for plant DNA isolation in teaching and research program.