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2002•Xibei Nong-Lin Keji Daxue xuebao. Ziran kexue banRequires access

Rapid method for extracting DNA from plant issues

Wei Li

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Abstract

Two methods were selected from various DNA isolated methods for extracting DNA from plant tissues rapidly.Through optimizing the steps,dosage of reagents and manipulating time,a reformative method is formed for extracting DNA rapidly.In our method,chloroform is replaced with liquid nitrogen,extracting reagent in which plant issues are skived was added,chloroformisoamyl alcohol is used to extract DNA repeatedly,high yield of DNA is gained.Using RNase,varified DNA could be obtained.The trait of the method is simple to apply in room temperature,timesaving (30 min ),less polluting,adaptable for extracting DNA from different plant materials and effective.

About this research paper

What this paper is about

Two methods were selected from various DNA isolated methods for extracting DNA from plant tissues rapidly.Through optimizing the steps,dosage of reagents and manipulating time,a reformative method is formed for extracting DNA rapidly.In our method,chloroform is replaced with liquid nitrogen,extracting reagent in which plant issues are skived was added,chloroformisoamyl alcohol is used to extract DNA repeatedly,high yield of DNA is gained.Using RNase,varified DNA could be obtained.The trait of the method is simple to apply in room temperature,timesaving (30 min ),less polluting,adaptable for extracting DNA from different plant materials and effective.

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Available abstract

Two methods were selected from various DNA isolated methods for extracting DNA from plant tissues rapidly.Through optimizing the steps,dosage of reagents and manipulating time,a reformative method is formed for extracting DNA rapidly.In our method,chloroform is replaced with liquid nitrogen,extracting reagent in which plant issues are skived was added,chloroformisoamyl alcohol is used to extract DNA repeatedly,high yield of DNA is gained.Using RNase,varified DNA could be obtained.The trait of the method is simple to apply in room temperature,timesaving (30 min ),less polluting,adaptable for extracting DNA from different plant materials and effective.

Key concepts: Isoamyl alcohol, DNA, Chloroform, DNA extraction, Reagent, Chromatography, Extraction (chemistry), Chemistry

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