Study on the detection of anti-pagumoganimus skrjabini antibody with DIGFA-kit and Dot-ELISA

OBJECTIVE To explore the sensitivity and specificity of detecting the anti-pagumoganimus skrjabini antibody by dot immunogold filtration assay(DIGFA)and dot enzyme-linked immunosorbent assay(Dot-ELISA).METHODS The DIGFA and Dot-ELISA were used to test the anti-P.skrjabini antibody in sera from the crowd in poular area and infected rats.RESULTS The positive rats of the antibody in sera from crowd in popular area and infected rats all were 5.1%、5.0 and 100%、97.5% respectively.Compared with the results of healthy big rats,big rats with trichinosis,rabbits with schistosomiasis and ascariasis cases,only one was positive with DIGFA in the rabbit of schistosomiasis.All of others in control group were negative.The antibody was detected at 2 weeks post-infectious in experimental animal.After 4 weeks,the positive rate reached 90% and lasted for 8 weeks.CONCLUSION The results show that these two methods have good sensitivity and specificity in detection of Pagumogonimus skrjabini antibodies.