2011•Zhongguo renshougonghuanbing zazhiRequires access
Detection of Pagumogonimus skrjabini antibody with DIGFA-kit and IEST
Song Ming-hua
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Abstract
The sensitivity and specificity of dot immunogold filtration asser(DIGFA) and IEST was explored in this study for detection of Pagumogonimus skrjabini antibody.The P.skrjabini antibody in sera from human and infected rats in endemic area were tested by using DIGFA and IEST methods.The positive rats of the antibody in sera from human and infected rats were 5.08% and 100% respectively.In the detection results of health big rats,big rats with trichinosis,rabbits with schistosomiasis,and human ascariasis cases,only one was positive with DIGFA in rabbits with schistosomiasis.The others in control group were negative.The antibody was detected out at 2 weeks post-infection in experimental animal,and at 4 weeks the positive rate reached 100% and lasted out 8 weeks.The results show that these two methods have good sensitivity and specificity in detection of Pagumogonimus skrjabini antibodies.