Comparison of two reagent kits for the rapid detection of Pagumogonimus skrjabini antibody.

Objective To explore the sensitivity and specificity of using a dot-immunogold filtration assay(DIGFA) and ELISA to detect the anti-Pagumogonimus skrjabini antibody.Methods DIGFA and ELISA were used to test for anti-P.skrjabini antibody in sera from the population in an area where paragonimiasis was prevalent and infected rats.Results Anti-P.skrjabini antibody was detected in sera from the population in an area where paragonimiasis was prevalent and infected rats at a rate of 5.08% and 100%,respectively.Compared to results from healthy rats,sera from rats with trichinosis,rabbits with schistosomiasis,and patients with ascariasis tested negative according to the two methods except for a positive result in DIGFA in a rabbit with schistosomiasis.The antibody was detected 2 weeks post-infection in lab animals at a rate of 20.00% and 27.50%,respectively,and at 4 weeks that rate reached 100% and remained so for 8 weeks.Conclusion The DIGFA reagent kit has the same sensitivity and specificity as ELISA.Specifically,it is rapid,easy to use,and inexpensive,so further use of this kit is warranted in areas where paragonimiasis is prevalent.