Study on the Isolated Culture Technique of Stem Apex of Huzhou Xueou

[Objective]The study aimed to provide reference for rapid propagation of seed lotus.[Method]With subterraneous stem apex of mainly cultivated lotus variety Chilaizao of Huzhou as explant,the sterilization effects of different sterilization time and methods(0.1%HgCl2 and 1.0%NaClO+0.1%HgCl2) on explants were investigated and the influence of sampling time and 6-BA and NAA concn.in medium on the differentiation of explants was studied.[Result]The sterilization effect of combination of HgCl2 and NaClO on explants was best.The optimum adventitious bud-induction medium was MS + 1.0 mg/L 6-BA + 0.2 mg/L NAA.The dormancy stage of subterraneous stem of lotus was optimum time for sampling and its differentiation rate of explants was highest.The regeneration buds were inoculated on the medium MS+1.0 mg/L 6-BA for multiplication,and then the bud seedlings with good growth potential were selected and inoculated on rooting medium MS+0.1 mg/L 6-BA+1.0 mg/L IBA +0.2%AC and they could root normally.[Conclusion] The optimum isolated culture conditions of subterraneous stem apex of lotus variety Chilaizao were confirmed in this study.