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Tissue Culture Techniques for Chrysanthemum

Gang Chen

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Abstract

The optimum medium formulation for bud induction,propagation and root generation for chrysanthemum were obtained by tissue culture experiments.The procedure was as follows: using flower buds as explants,the multiple shoots were induced in MS+BA 0.1 mg/L medium,propagated in MS+BA 0.1 mg/L,then the roots were generated and seedlings were strengthened in 1/2MS+NAA 0.1 mg/L medium,finally,seedlings for production were produced.

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The optimum medium formulation for bud induction,propagation and root generation for chrysanthemum were obtained by tissue culture experiments.The procedure was as follows: using flower buds as explants,the multiple shoots were induced in MS+BA 0.1 mg/L medium,propagated in MS+BA 0.1 mg/L,then the roots were generated and seedlings were strengthened in 1/2MS+NAA 0.1 mg/L medium,finally,seedlings for production were produced.

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Available abstract

The optimum medium formulation for bud induction,propagation and root generation for chrysanthemum were obtained by tissue culture experiments.The procedure was as follows: using flower buds as explants,the multiple shoots were induced in MS+BA 0.1 mg/L medium,propagated in MS+BA 0.1 mg/L,then the roots were generated and seedlings were strengthened in 1/2MS+NAA 0.1 mg/L medium,finally,seedlings for production were produced.

Key concepts: Explant culture, Tissue culture, Shoot, Botany, Biology, Horticulture, Murashige and Skoog medium, In vitro

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