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2007•Zhongguo shiyan fangjixue zazhiRequires access

Determination of Icariin in Ronggu Granule by HPLC

Xiumei Zhang

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Abstract

Objective:To determine the content of Icariin in Ronggu Granule by HPLC for quality control.Methods:Zorbax-SB-C_(18)(5 μm,4.6×150 mm) column was used with methanol∶water(60∶40)as mobile phase,and the determination wavelength was set at 270 nm.Results:The linear range was 0.1～1.0 μg for Icariin(r=(0.999 9)),the average recovery was 101.7% and RSD was 1.86%(n=5).Conclusion:This method is simple,reliable and accurate for the determination of icariin,it can be used for quality control of this preparation.

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What this paper is about

Objective:To determine the content of Icariin in Ronggu Granule by HPLC for quality control.Methods:Zorbax-SB-C_(18)(5 μm,4.6×150 mm) column was used with methanol∶water(60∶40)as mobile phase,and the determination wavelength was set at 270 nm.Results:The linear range was 0.1～1.0 μg for Icariin(r=(0.999 9)),the average recovery was 101.7% and RSD was 1.86%(n=5).Conclusion:This method is simple,reliable and accurate for the determination of icariin,it can be used for quality control of this preparation.

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Available abstract

Objective:To determine the content of Icariin in Ronggu Granule by HPLC for quality control.Methods:Zorbax-SB-C_(18)(5 μm,4.6×150 mm) column was used with methanol∶water(60∶40)as mobile phase,and the determination wavelength was set at 270 nm.Results:The linear range was 0.1～1.0 μg for Icariin(r=(0.999 9)),the average recovery was 101.7% and RSD was 1.86%(n=5).Conclusion:This method is simple,reliable and accurate for the determination of icariin,it can be used for quality control of this preparation.

Key concepts: Icariin, Chromatography, Granule (geology), High-performance liquid chromatography, Chemistry, Content determination, Linear range, Materials science

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