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2006•Chinese Journal of Veterinary DrugRequires access

Determination of Icariin in Cuyun Guanzhuye by HPLC

Zhu Yong-lin

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Abstract

A HPLC method was established for the determination of icariin in Cuyun Guanzhuye.HPLC analysis was carried out on Shim-Pack VP-ODS column(150 mm×4.6 mm,5 μm) with methanol-water(55∶45) as mobile phase at a flow rate of 1mL/min and UV detector at 270 nm.The calibration curve was in good linearity of icariin over the range of 0.1~4 μg(r=1.000).The average recovery was 99.9% with RSD of 2.4%(n=6).The method was sensitive,accurate and can be used to control the quality of Cuyun Guanzhuye.

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What this paper is about

A HPLC method was established for the determination of icariin in Cuyun Guanzhuye.HPLC analysis was carried out on Shim-Pack VP-ODS column(150 mm×4.6 mm,5 μm) with methanol-water(55∶45) as mobile phase at a flow rate of 1mL/min and UV detector at 270 nm.The calibration curve was in good linearity of icariin over the range of 0.1~4 μg(r=1.000).The average recovery was 99.9% with RSD of 2.4%(n=6).The method was sensitive,accurate and can be used to control the quality of Cuyun Guanzhuye.

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Available abstract

A HPLC method was established for the determination of icariin in Cuyun Guanzhuye.HPLC analysis was carried out on Shim-Pack VP-ODS column(150 mm×4.6 mm,5 μm) with methanol-water(55∶45) as mobile phase at a flow rate of 1mL/min and UV detector at 270 nm.The calibration curve was in good linearity of icariin over the range of 0.1~4 μg(r=1.000).The average recovery was 99.9% with RSD of 2.4%(n=6).The method was sensitive,accurate and can be used to control the quality of Cuyun Guanzhuye.

Key concepts: Icariin, Chromatography, High-performance liquid chromatography, Calibration curve, Chemistry, Analytical Chemistry (journal), Detection limit, Medicine

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