Effects of 8-Br-cAMP alone or combined with quercetin on Bcl-2, Bax, XRCC1 in Eca-109 cells

Aim:To study the effects of 8 Br cAMP combined with quercetin on apoptosis and apoptotic related proteins, including Bcl 2, Bax, and XRCC1 in Eca 109 cells. Methods: The cultured Eca 109 cells randomized into 4 groups: 8 Br cAMP(Br) group, quercetin (Q) group, (Br+Q) group,and Control (C) group treated with no any drugs, all cultured 48 h.Two kinds of specimens including cell slide and NCM specimens were prepared and Bcl 2 IR, Bax IR,and XRCC1 IR were detected by immunohistochemistry and the immunodot blot technique. Besides, the cell apoptosis was detected with TUNEL method in each group. Results: The cell apoptotic percentage was 70% in (Br+Q) group,42% in Br group, 35% in Q group, and 4% in C group. (Br+Q)Br or QC, P 0.001. When compared with C group, Bcl 2 IR and XRCC1 IR were decreased and Bax IR increased in the other 3 groups, P 0.001, Br vs Q or Br vs Q+Br, P 0.05. There was positive correlation between Bcl 2 IR and XRCC1 IR, but negative correlated with Bax IR, P 0.01. Conclusion: The Br combined with Q or single Br or Q alone could down regulate the expression of Bcl 2 and XRCC1, and up regulate that of Bax. (Br+Q) with highest cell apoptosis suggest that the traditional medicine, quercetin, combined with 8 Br cAMP treatment may be more efficient in clinic.