Differentiation of Eca-109 cell line induced by 8-Br-cAMP

Aim: To explore the effects of 8 Br cAMP on differentiation of human esophageal cancer Eca 109 cell line.Methods: The cultured Eca 109 cells were divided into 2 groups: Br group: cells were cultured with 2×10 -5 mol/L 8 Br cAMP for 24 h; Control group: cells were cultured without 8 Br cAMP for 24 h. The in situ hybridization, immunohistochemistry and RNA and protein dot blot technique were used to study the effects of 8 Br cAMP on the expression of genes c myc, wtp53, iNOS, and EGFR IR in each group. Results: In situ hybridization signal and immunohistochemistry IR were all localized in the cytoplasm. The scanning values showed that: a): c myc mRNA: Br group was 3.38±0.99,C group was 5.18±1.39;b): wtp53 mRNA: Br group was 2.74±0.83,C group was 0.38±0.27;c): iNOSmRNA: Br group was 4.52±0.74,C group was 2.63±0.13;d): EGFR IR: Br group was 2.37±1.05,C group was 4.38±0.48; P 0.05～0.01. Conclusion: c myc, wtp53, and NO may be involved in differentiation of Eca 109 cell induced by 8 Br cAMP.