Effects of 8-Br-cAMP ar quercetin combined use on changes of mutator phenotypes in Eca-109 cells

Objective: To study the effects of 8-Br-cAMP and quercetin alone or combined use on changes of mutator phenotypes in Eca-109 cells.Methods: The cultured Eca-109 cells randomized into 4 groups:8-Br-cAMP(Br) group,quercetin(Q) group,(Br+Q) group,and Control(C) group treated with no any drugs.After cultured 48 h,two kinds of specimens including cell slide and NCM specimens were prepared and Bcl-2-IR,Bax-IR,XRCC1-IR,MRP and POLB were detected by immunohistochemistry and the immunodot blot technique.Besides,the cell apoptosis was detected with TUNEL method in each group.Results: The cell apoptotic percentage was 70% in(Br+Q) group,42 % in Br group,35 % in Q group,and 4 % in C group.(Br+Q)Br or QC(P0.001).When compared with C group,Bcl-2-IR and XRCC1-IR were decreased and Bax-IR increased in the other 3 groups(P0.001),Br vs Q or Br vs(Q+Br)(P0.05).There waspositive correlation between Bcl-2-IR and XRCC1-IR,but negative correlated with Bax-IR(P0.01);The immunoreactivity(IR) showed:MRP-IR,CBr,Q or (Br+Q) group(P0.01);POLB-IR,CBr,Q or(Br+Q) group(P0.01).There was a signify cant positive correlation between MRP and POLB.Conclusion: The Br combined with Q or single Br or Q lone could down regulate the expression of Bcl-2,XRCC1,MRP and POLB and up-regulate that of Bax,(Br+Q) with highest cell apoptosis,which exhibites reduction of the mutator phenotype.