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2005•Journal of Jilin Institute of Chemical TechnologyRequires access

Study on Assay of tetrahydropalmatine in Corydalis yanhusuo W.T.Wang by HPLC

Meng Xiang-jin

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Abstract

To determine the content of tetrahydropalmatine in Corydalis yanhusuo W.T.Wang.HPLC method was selected to determine the content.The separation was performed on an analytical columm φ4.6×250mm Hypersil BDS C18.The UV detector was set at 230nm.The mobile phase was methanol-water(pH=8.2)=65∶35,1.0mL/min.The method is orecise with RSD=2%.The average recovery was 98.05%～100%.The liner range was 0.29～048μg.The method was proved to be simple and reliable,and can be used for the quality control of yanhusuo.

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What this paper is about

To determine the content of tetrahydropalmatine in Corydalis yanhusuo W.T.Wang.HPLC method was selected to determine the content.The separation was performed on an analytical columm φ4.6×250mm Hypersil BDS C18.The UV detector was set at 230nm.The mobile phase was methanol-water(pH=8.2)=65∶35,1.0mL/min.The method is orecise with RSD=2%.The average recovery was 98.05%～100%.The liner range was 0.29～048μg.The method was proved to be simple and reliable,and can be used for the quality control of yanhusuo.

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Available abstract

To determine the content of tetrahydropalmatine in Corydalis yanhusuo W.T.Wang.HPLC method was selected to determine the content.The separation was performed on an analytical columm φ4.6×250mm Hypersil BDS C18.The UV detector was set at 230nm.The mobile phase was methanol-water(pH=8.2)=65∶35,1.0mL/min.The method is orecise with RSD=2%.The average recovery was 98.05%～100%.The liner range was 0.29～048μg.The method was proved to be simple and reliable,and can be used for the quality control of yanhusuo.

Key concepts: Tetrahydropalmatine, Chromatography, High-performance liquid chromatography, Corydalis, Chemistry, Medicine, Traditional Chinese medicine, Alternative medicine

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