2008•China Forestry Science and TechnologyRequires access
Establishment of an Efficient Regeneration System for Populus tomentosa Clone-85
Li Ling
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Abstract
To optimize the regeneration system of P. tomentosa Clone-85,influences of the different medium and proportion of hormone on adventitious bud and root for leaf-explants were studied by uniform design and orthogonal experimental design.The results demonstrated that the optimal medium and proportion of hormone was MS+0.5 mg/L 6-BA+0.01 mg/L NAA for bud differentiation,and1/2MS+0.05 mg/L NAA+0.05 mg/L IBA for adventitious root.Using the optimal regeneration system of P.tomentosa Clone85,the average 21.2 buds was induced in each leaf,the rooting rate was 99%, and the average root number was 7.The results laid a sound foundation for regeneration of P.tomentosa 85 in industry and gene transformation by leaf disc method.