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INDUCTION,DIFFERENTIATION AND PLANT REGENERATION OF TRIPLOID CALLI OF POPULUS TOMENTOSA

Yi Li, He Mingzhu, Ma Haiyun

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Abstract

Regenerating plants were produced from immature leaves of the triploid of Populus tomentosa by tissue culture on MS basic medium with different plant hormone. The result showed that callus induction frequency reached to 87.6 percent on MS+6 BA 0.5 mg/L + NAA0.1 mg/L ; differentiation frequency reached to 68.5 percent on 1/2 MS + IAA0.1 mg/L + 6 BA0.1. It can be generated from one to 7.4 axillary shoots on MS+6 BA0.5 mg/L +NAA0.01 mg/L . Rooting frequency of the shoots got to 85.7 percent on MS+ IBA 1.0mg/L

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What this paper is about

Regenerating plants were produced from immature leaves of the triploid of Populus tomentosa by tissue culture on MS basic medium with different plant hormone. The result showed that callus induction frequency reached to 87.6 percent on MS+6 BA 0.5 mg/L + NAA0.1 mg/L ; differentiation frequency reached to 68.5 percent on 1/2 MS + IAA0.1 mg/L + 6 BA0.1. It can be generated from one to 7.4 axillary shoots on MS+6 BA0.5 mg/L +NAA0.01 mg/L . Rooting frequency of the shoots got to 85.7 percent on MS+ IBA 1.0mg/L

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Available abstract

Regenerating plants were produced from immature leaves of the triploid of Populus tomentosa by tissue culture on MS basic medium with different plant hormone. The result showed that callus induction frequency reached to 87.6 percent on MS+6 BA 0.5 mg/L + NAA0.1 mg/L ; differentiation frequency reached to 68.5 percent on 1/2 MS + IAA0.1 mg/L + 6 BA0.1. It can be generated from one to 7.4 axillary shoots on MS+6 BA0.5 mg/L +NAA0.01 mg/L . Rooting frequency of the shoots got to 85.7 percent on MS+ IBA 1.0mg/L

Key concepts: Shoot, Callus, Botany, Biology, Regeneration (biology), Axillary bud, Tissue culture, Murashige and Skoog medium

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INDUCTION,DIFFERENTIATION AND PLANT REGENERATION OF TRIPLOID CALLI OF POPULUS TOMENTOSA — Research Paper | ScholarLens