Research of Establishment of Tissue Culture Regeneration System About Two New Hybrid Poplars

Populus tomentosa× Populus adenopoda and Populus tomentosa × 84K poplar were two new hybrids.Sterile shoots of them were used in this tissue culture study as explants.Research on the potential of plantlet regeneration was compared in order to filter out a more satisfactory bud proliferation and rooting medium,establish effective ways to the stem-proliferation of fast propagation.The results showed that the best multiplication medium for Populus tomentosa × Populus adenopoda was MS +6-BA 0.5 mg/L + NAA 0.01 mg/L and the shoots proliferation rate was 93.3%,the average number of proliferating stem axillary buds was 6.38.And for the Populus tomentosa× 84K poplar was MS +6-BA 1.0 mg/L + NAA 0.01 mg/L and the shoots proliferation rate was 90.0%,the average number of proliferating stem axillary buds was 6.00.The best rooting medium for Populus tomentosa × Populus adenopoda was MS + NAA 0.15 mg/L,rooting rate reached 86.7%,and the Populus tomentosa × 84K was MS + NAA 0.15 mg/L,rooting rate was 96.7 %.