2011•Zhongguo shiyan fangjixue zazhiRequires access
Effects of Shugan Jianpi Recipe on 5-Hydroxytryptamine,Calcitonin Gene Related Peptide in Rats with Diarrhea-predominant Irritable Bowel Syndrome
Bin Han
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Abstract
Objective:To observe the effect of Shugan Jianpi Recipe on 5-hydroxytryptamine(5-HT),calcitonin gene related peptide(CGRP) in rats with diarrhea predominant irritable bowel syndrome(D-IBS).Method: Sixty Wistar rats were randomized into normal group,model group,low,middle and high-dose groups of Shugan Jianpi Recipe,and dicetel group.Cold-binding applications were given to establish similar model of D-IBS.Violet spectrophotometer was used for determining the levels of 5-HT in serum.RIA was used for measuring the levels of CGRP in serum.The expressions of 5-HT and CGRP in intestinal and colon mucosa and colon tissue were detected by immunohistochemical SABC method.Result: In the model group,the 5-HT level increased,CGRP decreased,compared with those in the normal group(P0.05).The Shugan Jianpi Recipe's middle,high-dose increased 5-HT and decreased CGRP significantly,compared with that in the model group(P0.01).Conclusion: The therapeutic mechanism of Shugan Jianpi Recipe for D-IBS is probably related with adjustment of 5-HT and CGRP in serum and colon tissue.