2014•Liaoning zhongyi zazhiRequires access
Effects of Shugan Decoction on SP and CGRP in Colon and DRG of Rats with Visceral Hypersensitivity
Shang Jingjua
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Abstract
Objective: To investigate the effects of Shugan Decoction on SP and CGRP in the colon and DRG of rats with visceral hypersensitivity. Methods: Ninety-six male Wistar rats were randomly divided into six groups: control group,model group,low dose Shugan Decoction group,middle dose Shugan Decoction group,high dose Shugan Decoction group and dicetel group. Rats were exposed to 1-h water avoidance stress( WAS) daily for 10 consecutive days. Shugan Decoction groups and dicetel group were given intragastric administration half an hour before WAS from the 4th day to the 10th day. The abdominal withdrawal reflex( AWR) to colorectal distension was assessed at the end of the 10-day period. Western-blotting method was applied to investigate the contents of SP and CGRP in the colon and DRG. Results: Compared with the normal group,SP and CGRP in the colon and DRG of model group were significantly increased. Compared with the model group,SP and CGRP in the colon and DRG of middle dose Shugan Decoction group,high dose Shugan Decoction group and dicetel group were significantly reduced. Conclusion: Shugan Decoction can reduce visceral hypersensitivity by regulating expression of SP and content of CGRP in the colon and DRG.