2012•Tianjin Journal of Traditional Chinese MedicineRequires access
Effects of Shugan Jianpi prescription on 5-HT,SP and VIP content in the plasma and colon of D-IBS rat
Shengsheng Zhang
Open publisher page 0 citations
Abstract
[Objective] To observe the effect of Shugan Jianpi prescription(SGJP) on the concentration of 5-HT,SP and VIP content in the plasma and colon of D-IBS rat.[Methods] Sixty SD male rats were randomly divided into six groups with ten rats each: normal group,D-IBS model group,pinaverium bromide group and SGJP low,middle,high dosage group.D-IBS rat model was induced by intracolonic instillation of acetic acid and restraint stress and immunohistochemistry technology was employed to test the 5-HT,SP and VIP content in the plasma and colon of rat.[Results] The defecation moisture was significantly reduced by the SGJP prescription(P0.05);the AWR scores of the D-IBS rats under different expansion volume were reduced after treated with the middle and high dosage of SGJP prescription(P0.05);the content of plasma 5-HT and colon SP of D-IBS rats was reduced after administration with SGJP prescription,but plasma SP,VIP and colon VIP were not influenced.[Conclusion] The SGJP prescription could improve the diarrhea and visceral hypersensitivity and regulate the content of 5-HT and SP,which shows that the SGJP prescription could efficiently treat D-IBS by regulating gut hormones.