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2005•Unpublished venueRequires access

Genotyping of Pseudomonas aeruginosa by Randomly Amplified Polymorphic DNA

Hai‐Ping Cheng

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Abstract

OBJECTIVE To monitor the hospital infection on genotyping Pseudomonas aeruginosa (PAE) with randomly amplified polymorphic DNA(RAPD) assay. METHODS Optimal reaction system and amplification condition of RAPD were used for genotyping of the 24 PAE strains isolated from the clinical samples. RESULTS The 24 PAE strains could be distinguished into 14 kinds of RAPD type, the type ability was 100%. There was not the same type of PAE in the different patients of same wards, and there was not the same type of PAE at the 3 wards. Five instances in 7 of PAE hospital infection had the same RAPD type, and 2 instances had jump-RAPD type without the same in (other′s). CONCLUSIONS RAPD could to be used in the molecular epidemiological study of PAE.

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What this paper is about

OBJECTIVE To monitor the hospital infection on genotyping Pseudomonas aeruginosa (PAE) with randomly amplified polymorphic DNA(RAPD) assay. METHODS Optimal reaction system and amplification condition of RAPD were used for genotyping of the 24 PAE strains isolated from the clinical samples. RESULTS The 24 PAE strains could be distinguished into 14 kinds of RAPD type, the type ability was 100%. There was not the same type of PAE in the different patients of same wards, and there was not the same type of PAE at the 3 wards. Five instances in 7 of PAE hospital infection had the same RAPD type, and 2 instances had jump-RAPD type without the same in (other′s). CONCLUSIONS RAPD could to be used in the molecular epidemiological study of PAE.

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Available abstract

OBJECTIVE To monitor the hospital infection on genotyping Pseudomonas aeruginosa (PAE) with randomly amplified polymorphic DNA(RAPD) assay. METHODS Optimal reaction system and amplification condition of RAPD were used for genotyping of the 24 PAE strains isolated from the clinical samples. RESULTS The 24 PAE strains could be distinguished into 14 kinds of RAPD type, the type ability was 100%. There was not the same type of PAE in the different patients of same wards, and there was not the same type of PAE at the 3 wards. Five instances in 7 of PAE hospital infection had the same RAPD type, and 2 instances had jump-RAPD type without the same in (other′s). CONCLUSIONS RAPD could to be used in the molecular epidemiological study of PAE.

Key concepts: RAPD, Genotyping, Biology, Genotype, Pseudomonas aeruginosa, Microbiology, DNA, Typing

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