Molecular Typing of Pseudomonas aeruginosa by Randomly Amplified Polymorphic DNA

Pseudomonas aerugionsa is a commonly isolated nosocomial pathogen. DNA fingerprinting of P. aerugionsa is examined by randomly amplified polymorphic DNA (RAPD). In this study, P. aeruginosa were isolated from environmental and clinical specimens and the molecular typing of the microorganisms was investigated by RAPD. Thirty strains of P. aeruginosa were selected from the strains isolated formerly and submitted for type identification to the University Hospital. 15 strains of P. aeruginosa were received from Chungnam University Hospital and 14 strains from Gyeongsang University Hospital. DNA of P. aeruginosa was extracted by Qiagen genomic DNA kit. PCR mixtures were set up and incubated. Reactions mixtures were made to be optimal for P. aeruginosa. RAPD typing analysis was carried out by the multivariate statistical program (MVSP) V3.0. RAPD type Ⅰ was the most common pattern and included 23 strains. Most of strains from Gyeongsang University Hospital belonged to RAPD type Ib and 15 strains from Chungnam University Hospital to RAPD type Ⅰ or Ⅱ. RAPD typing of P. aeruginosa isolated from the environmental and clinical specimens was very simple and reproducible.