2010•The Chinese Journal of Modern Applied PharmacyRequires access
The Effect of Kanglaite Injection on Expression of Cyclin Modulin P16,P63 in HL-60/ADR Cells
LI Ji-rong
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Abstract
OBJECTIVE To investigate the effect of Chinese medicine Kanglaite injection on expression of cyclin modulin P16,P63 in HL-60/ADR cells.METHODS HL-60/ADR cells during logarithmic growth phase were treated with Kanglaite injection,growth curve was based on cell counting;the inhibitory effect of adriamycin(ADR) on Kanglaite injection-treated HL-60/ADR cells was tested by MTT assay;the concentration of intracellular adriamycin and the expression of P16,P63 were detected by flow cytometry.RESULTS In each group,live cell count reached a peak on the 6th day,compared with control group,the Kanglaite injection-treated cell count was lower with statistical significance(P0.05).Treated HL-60/ADR cells with 1 mg·mL-1 Kamglaite for 96 h,the inhibitory effect of adriamycin on the cells were greater than that of control groups(P0.05).After treatment with different concentration of Kanglaite injection,the intrancellular ADR fiourescence of 1.5 μg·mL-1 ADR-treated for 6 h were statistical higher than that of control group(P0.05).Treated HL-60/ADR cells with 1 mg·mL-1 Kamglaite for 48 h,compared with the control group,the expression of P16,P63 were all significantly increased(P0.05).CONCLUSION HL-60/ADR cells exposed to Kanglaite injection resulted in dose-depentent inhibitory,Kanglaite injection could increase the sensitivity of HL-60/ADR cells to ADR.Kanglaite injection could increase the expression level of P16,P63 in HL-60/ADR cells,that maybe involved in the anti-tumor mechanics of Kanglaite injection.