2006•The Practical Journal of CancerRequires access
Reversal of Multi-drug Resistance in HL-60/ADR Cells by Chinese Medicine Kanglaite
LI Ji-ron
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Abstract
Objective To explore the reversal efficacy of Chinese Medicine Kanglaite on mutidrug resistance(MDR) cells HL-60/ADR and it’s mechanism.Methods HL-60/ADR cells at logarithmic growth phase were treated with Kanglaite,the inhibitory effect of Adrimay(ADR) on kanglaite-treated HL-60/ADR cells were tested by MTT assay.The concentration of intracellular ADR and the expression level of P-170 were detected by flow cytometry.Results After 1mg/ml kamglaite treated HL-60/ADR cells for 96 h,The inhibitory effect of ADR on the cells are statistical higher than that of no-kanglaite-treated groups,P0.05.After kanglaite treatment at different concentration,the intrancellular ADR fiourescence of 1.5 μg/ml ADR-treated for 6h are statistical higher than that of control group,P0.05.1 mg/ml Kanglaite treated HL-60/ADR for 48h,the expression of P-170 was significantly decrease.P0.05.Conclusion Kanglaite could increase intracellular concentrion of ADR and decrease the expression level of P-170 on HL-60/ADR cells so that the sensitivity of HL-60/ADR cells to ADR was increased significantly.