2007•Acta Academiae Medicinae ZunyiRequires access
The effect of chinese medicine kanglaite on expression of cyclinA in HL-60/ADR cells
LI Ji-rong
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Abstract
Objective To investigate the effect of Chinese Medicine Kanglaite on expression of cyclinA in HL-60/ADR cells.Methods HL-60/ADR cells at logarithmic growth phase were treated with Kanglaite,the inhibitory effect of Adrimay(ADR) on kanglaite-treated HL-60/ADR cells were tested by MTT assay.The intracellular ADR fiourescence of certain concentration ADR-treated for certain time were tested by flow cytometry.The various phases of cell cycle and the expression level of cyclinA were detected by flow cytometry.Results(1) After 1mg/ml kamglaite treated HL-60/ADR cells for 96h,The inhibitory effect of ADR on the cells are statistically higher than that of no-kanglaite-treated groups,P0.05.(2) After kanglaite treatment at different concentration,The intrancellular ADR fiourescence of 1.5ug/ml ADR-treated for 6h are statistically higher than that of control group,P0.05.(3)1mg/ml Kanglaite treated HL-60/ADR for 48h,the expression of cyclinA was significantly increase.P0.05.Compared with the control group,the various phases of cell cycle were not significant.Conclusions Kanglaite could increase intracellular concentration of ADR and the expression level of cyclinA on HL-60/ADR cells so that the sensitivity of HL-60/ADR cells to ADR was increased significantly.