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2009•Journal of Pediatric PharmacyRequires access

Determination of Baicalin in Bikangbao Capsule by HPLC

Yajun Zhang

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Abstract

Objective:To establish a method for determination of baicalin in Bikangbao capsule by HPLC. Methods: HPLC was performed on Kromasil C18 column with methano1-water-phosphonic acid (40∶60∶0.2) as mobile phase. The UV detection wavelength was 280 nm. Results: The linear range of baicalin was sample size 5.2～83.2 μg/mL (r=0.9998), the average recovery was 97.8%. Conclusions: The method was simple, accurate and fast, and could be used for the determination of baicalin in Bikangbao capsule.

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What this paper is about

Objective:To establish a method for determination of baicalin in Bikangbao capsule by HPLC. Methods: HPLC was performed on Kromasil C18 column with methano1-water-phosphonic acid (40∶60∶0.2) as mobile phase. The UV detection wavelength was 280 nm. Results: The linear range of baicalin was sample size 5.2～83.2 μg/mL (r=0.9998), the average recovery was 97.8%. Conclusions: The method was simple, accurate and fast, and could be used for the determination of baicalin in Bikangbao capsule.

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Available abstract

Objective:To establish a method for determination of baicalin in Bikangbao capsule by HPLC. Methods: HPLC was performed on Kromasil C18 column with methano1-water-phosphonic acid (40∶60∶0.2) as mobile phase. The UV detection wavelength was 280 nm. Results: The linear range of baicalin was sample size 5.2～83.2 μg/mL (r=0.9998), the average recovery was 97.8%. Conclusions: The method was simple, accurate and fast, and could be used for the determination of baicalin in Bikangbao capsule.

Key concepts: Baicalin, Capsule, Chromatography, High-performance liquid chromatography, Linear range, Medicine, Chemistry, Detection limit

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