2010•Shijie zhongxiyi jiehe zazhiRequires access

Determination of baicalin in Anfei capsule by HPLC.

Xiangping Pei, Shurong Zhang, Pei Miao-rong, Yufeng Yang, Haifeng Wang

Abstract

Objective To determine the content of baicalin in Anfei capsule by HPLC.Methods Chromatographic column:Diamonsil C18 column(4.6 mm×150 mm,5 μm);mobile phase:methol-water-phosphoric acid(47∶52∶0.2);flow rate:1.0 mL·min-1;detection wavelength:280 nm;column temperature:ambient temperature.Results The linear relation of baicalin was good in the range from 7.5 μg to 60 μg.Regression equation:y=63.482x+0.644 7;r=0.999 5.Average recovery was 99.8%.Conclusion The method is simple,rapid and accurate in operation and it is suitable for the determination of content of baicalin in Anfei capsule.

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Available abstract

Key concepts: Baicalin, Chromatography, Capsule, Phosphoric acid, High-performance liquid chromatography, Content determination, Linear range, Medicine