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Quantitative Determination of Baicalin in Yanreqing Capsule by HPLC

Zhao Li, LI Qiao-ru

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Abstract

OBJECTIVE:To establish a HPLC method for the quantitative determination of Baicalin in Yanreqing capsule. METHODS:HPLC was performed on Hypersil BDS C18(150mm×4.6mm,5μm) with column at room temperature. The mobile phase consisted of methanol-water-phosphoric acid(47∶53∶0.2) at a flow rate of 1.0mL·min-1.The detection wavelength was set at 280nm. RESULTS:The linearity of Baicalin was good in the range of 0.101～1.01μg(r=0.999 6) and the average recovery rate is 99.67% with RSD at 0.82%(n=9).CONCLUSION:The method is simple, accurate, sensitive and reproducible, and it can be applied for the quality control of Yanreqing capsule.

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What this paper is about

OBJECTIVE:To establish a HPLC method for the quantitative determination of Baicalin in Yanreqing capsule. METHODS:HPLC was performed on Hypersil BDS C18(150mm×4.6mm,5μm) with column at room temperature. The mobile phase consisted of methanol-water-phosphoric acid(47∶53∶0.2) at a flow rate of 1.0mL·min-1.The detection wavelength was set at 280nm. RESULTS:The linearity of Baicalin was good in the range of 0.101～1.01μg(r=0.999 6) and the average recovery rate is 99.67% with RSD at 0.82%(n=9).CONCLUSION:The method is simple, accurate, sensitive and reproducible, and it can be applied for the quality control of Yanreqing capsule.

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Available abstract

OBJECTIVE:To establish a HPLC method for the quantitative determination of Baicalin in Yanreqing capsule. METHODS:HPLC was performed on Hypersil BDS C18(150mm×4.6mm,5μm) with column at room temperature. The mobile phase consisted of methanol-water-phosphoric acid(47∶53∶0.2) at a flow rate of 1.0mL·min-1.The detection wavelength was set at 280nm. RESULTS:The linearity of Baicalin was good in the range of 0.101～1.01μg(r=0.999 6) and the average recovery rate is 99.67% with RSD at 0.82%(n=9).CONCLUSION:The method is simple, accurate, sensitive and reproducible, and it can be applied for the quality control of Yanreqing capsule.

Key concepts: Baicalin, Chromatography, Phosphoric acid, Capsule, High-performance liquid chromatography, Chemistry, Content determination, Biology

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