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2011•Strait Pharmaceutical JournalRequires access

Determination of baicalin in Fenciping capsule by HPLC

Luo Shi-rong

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Abstract

OBJECTIVE To establish a method for determination of baicalin in Fenciping Capsule.METHODS Using a HPLC method with Hypersil BDS C18(4.6×250mm,5μm)column,the mobile phase was a methanol-0.2%phosphoric acid(39∶61)the detection wavelength at 280nm,flow rate was 1.0mL·min-1,and column temperature was 30℃.RESULTS The precision and stability were fine,The standard curve of baicalin was rectilinear within the range of 3.408～20.448μg·mL-1,r=1.The average recovery of baicalin was 100.09% with RSD of 1.55%.CONCLUSION The method was simple,accurate and reproducible,and can be used for the determination of baicalin.

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OBJECTIVE To establish a method for determination of baicalin in Fenciping Capsule.METHODS Using a HPLC method with Hypersil BDS C18(4.6×250mm,5μm)column,the mobile phase was a methanol-0.2%phosphoric acid(39∶61)the detection wavelength at 280nm,flow rate was 1.0mL·min-1,and column temperature was 30℃.RESULTS The precision and stability were fine,The standard curve of baicalin was rectilinear within the range of 3.408～20.448μg·mL-1,r=1.The average recovery of baicalin was 100.09% with RSD of 1.55%.CONCLUSION The method was simple,accurate and reproducible,and can be used for the determination of baicalin.

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Available abstract

OBJECTIVE To establish a method for determination of baicalin in Fenciping Capsule.METHODS Using a HPLC method with Hypersil BDS C18(4.6×250mm,5μm)column,the mobile phase was a methanol-0.2%phosphoric acid(39∶61)the detection wavelength at 280nm,flow rate was 1.0mL·min-1,and column temperature was 30℃.RESULTS The precision and stability were fine,The standard curve of baicalin was rectilinear within the range of 3.408～20.448μg·mL-1,r=1.The average recovery of baicalin was 100.09% with RSD of 1.55%.CONCLUSION The method was simple,accurate and reproducible,and can be used for the determination of baicalin.

Key concepts: Baicalin, Chromatography, Phosphoric acid, High-performance liquid chromatography, Capsule, Chemistry, Organic chemistry, Botany

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