Extracting the bioactive-products from the wastes of vint grape

Protein, fat, fibre, carbohydrate, total phenol, proanthocyanidin et al, in the skin, stem and seed of vint grape, has been analyzed. The percentage of total phenol in the skin, stem and seed of vint grape is 1.82%, 1.79%, 4.85%(calculate as GAE), the percentage of proanthocyanidin is 0.85%, 0.72%, 3.05% (10% moisture of raw material). It shows that the extract ratio of total phenol is the highest of 100.02% when the grape seed powder of 130~150 mesh is extracted by 90% methanol with stirring 40min at 20℃; The extract ratio of proanthocyanidin is the highest of 99.11% when 70% acetone is introduced. At the thermostatic bath, the more power of ultrasonic wave, the higher extract ratio of total phenol and proanthocyanidin. The extract ratio of total phenol from grape seed with 80~150 mesh drying by vacuum is 9.84% higher than drying by nature; the proanthocyanidin is 12.61% higher.