Study on the Extraction of Proanthocyanidin from Grape Seeds

Through the digestion method proanthocyanidin was extracted from grape seeds.The influence of single factor on the digestion effect such as different solvent,digestion time,extraction temperature,volume fraction of ethanol the ratio of grape seeds weight to solvent amount and so on were investigated,and the best range of each factor was determined.Moreover,the optimal technique conditions were obtained through the orthogonal experimental design:the volume fraction of ethanol was 70%,extraction temperature was 50℃,the ratio of material to solvent was 1∶7(g/mL),extraction time was 30min,and the extraction frequency was 3 times.