The Cloning and Sequence Analysis of the end of 3'cDNA of Mongolia Sheep Bcl-2 Gene

In order to amplify the end of 3'of the Bcl-2 gene of Mongolia sheep,we designed two pair of primers according to the reported Bos taurus Bcl-2 gene sequence in the GenBank.Total RNA was extracted from the spleen of Mongolia sheep and the cDNA encoding Mongolia sheep bcl-2 was amplified by the reverse transcription PCR(RT-PCR).The purified RT-PCR product was cloned in pBlueselect T vector.The results of restriction endonuclease pattern analysis of recombinant plasmid and DNA sequencing demonstrated that the Bcl-2 is belong to the family of Bcl-2 because the cDNA contain a open reading frame(ORF) of 261 bases which encoded 83 amino acid.The nucleotide sequences of Mongolia sheep Bcl-2 gene were compared with the counterpart sequences of Bos taurus,and the nucleotide homology was 95.8%.