Identification for Genetically Modified Rape RT73 Line with SYBR~ Green I Real Time PCR

The genetically modified rape RT73 line with commerce planting was identified by applying SYBR Green I real-time PCR assays and using specific primers.The results showed that the SYBR Green I could selectively combine the characteristic of double chain DNA and identify fluorescence signal produced by sequence amplification of specific target of RT73 line,and confirm the melting point of(80.2±0.5) ℃ by melting curve,while the fluorescence signal in other rape lines didn't be detected.The SYBR Green I real-time PCR could availably differentiate special or non-special products and primer dimers by melting curve,which is a new method for gene idenfification.