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Detection for Bt transgenic rice with SYBR~ Green I real time PCR

Yong Liu

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Abstract

Bt genetically modified rice was identified with SYBR~ Green Ⅰ real-time PCR assays and three pairs of specific primers to establish a new detection technique for GMO.The results showed that the SYBR~ Green Ⅰ could selectively combine the characteristic of double chain DNA and identify fluoresce signal produced by sequence amplification of specific target of Bt rice(CaMV35S,NOS,Cry1Ab/c).The SYBR~ Green Ⅰ real-time PCR could availably differentiate special or non-special products and primer dimers by melting curve,which is a new method for GMO detection.

About this research paper

What this paper is about

Bt genetically modified rice was identified with SYBR~ Green Ⅰ real-time PCR assays and three pairs of specific primers to establish a new detection technique for GMO.The results showed that the SYBR~ Green Ⅰ could selectively combine the characteristic of double chain DNA and identify fluoresce signal produced by sequence amplification of specific target of Bt rice(CaMV35S,NOS,Cry1Ab/c).The SYBR~ Green Ⅰ real-time PCR could availably differentiate special or non-special products and primer dimers by melting curve,which is a new method for GMO detection.

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Available abstract

Bt genetically modified rice was identified with SYBR~ Green Ⅰ real-time PCR assays and three pairs of specific primers to establish a new detection technique for GMO.The results showed that the SYBR~ Green Ⅰ could selectively combine the characteristic of double chain DNA and identify fluoresce signal produced by sequence amplification of specific target of Bt rice(CaMV35S,NOS,Cry1Ab/c).The SYBR~ Green Ⅰ real-time PCR could availably differentiate special or non-special products and primer dimers by melting curve,which is a new method for GMO detection.

Key concepts: SYBR Green I, Melting curve analysis, Primer (cosmetics), Real-time polymerase chain reaction, Genetically modified rice, Primer dimer, Polymerase chain reaction, Molecular biology

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