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2001•Nature GeneticsOpen access

Quantification and normalization of gene expression using SYBR Green I real-time RT-PCR

Jo Vandesompele, Johanna Iso-Oja, Anne De Paepe, Frank Speleman

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Key concepts: Biology, Normalization (sociology), Real-time polymerase chain reaction, Computational biology, Genetics, Gene, SYBR Green I, Gene expression profiling

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