Identification for Genetically Modified Canola RT73 Line with SYBR~ Green I Real Time PCR

To identify genetically modified(GM) canola RT73 line,a SYBR Green I real-time PCR assay was performed in this study.Specific primers for inserted genes in the RT73 were used to conduct the SYBR Green I real-time PCR assays.The SYBR Green I real-time PCR method was established to detect and identify GM canola lines.The endogenesis FatA gene of canola could be designed to control the testing process and avoid the fake negative result.The results showed that the SYBR Green I could selectively combine double chain DNA,identify RT73 canola,and confirm melting point of 80.2(±0.5) by melting curve,while other GM and NO-GM canola didn't be detected.The SYBR Green I real-time PCR could be a new method for detecting genetically modified(GM) canola RT73 line.