Cloning and sequence analysis of BC gene from Camellia oleifera

With the near-matured seeds of 'Xianglin No.1' of Camellia oleifera as material to extract total RNA,the whole length cDNA of BC gene that is a subunit of ACCase from Camellia oleifera was obtained by means of the techniques such as degenerate PCR,RACE and the overlap extension PCR.The full-length of cDNA sequence of BC gene is 1 901 bp,and has a 1 599 bp ORF coding 533 amino acids.The pI value of BC protein is 6.88,the molecular mass of the protein is 58 509.3 u,the protein bears two trans-membrane domain,has no signal peptide,and is a unstable protein.There are several pro-sites in BC,such as N-glycosylation site,Protein kinase C phosphorylation site,ATP-grasp fold profile and so on.In the three-dimensional model of BC,we found 12 alpha helixes and a cupped area in the central of this protein.The gene was submitted to the GeneBank and named co-bc.