Cloning and Sequence Analysis of FBPase Gene from Camellia oleifera

FBPase is one of the key enzymes controlling Calvin Cycle,Which helps to improve Clavin circulation to accelerate photosynthetic efficiency of plants.The full length cDNA of FBPase gene was separated from the EST library and the cDNA library of Camellia oleifera by the method of 5'RACE and 3'RACE.The results showed that this gene is 1 356 bp in length,and it has an open reading frame of 1 020 bp,and the alignment of nucleotide acids sequence reveals that the comparability of Camellia oleifera and populus trichocarpa is 84%.This gene encodes 339 aminoacids,the protein is a stable protein whose Isoelectric point is 5.54.Camellia oleifera FBPase encoded protein does not contain disulfide,thus the gene is c-FBPase named co-fbp.This study is significant help to the research on the photosynthesis of Camellia oleifera,increaseing energy efficiency and production.