Cloning and sequence analysis of full-length cDNA of LOX gene from Camellia oleifera

In order to reveal the role of LOX gene in the development of Camellia oleifera seeds and the impact on seeds oil quality,a full-length cDNA of the LOX gene was cloned from C.oleifera seeds on the basis of transcriptome library with RT-PCR and RACE methods.The bioinformatics analysis suggested that the 2 698 bp cDNA was composed of a 2 406 bp ORF,a 161bp 5'UTR and a 131bp 3'UTR that contained a 16 bp polyA.The characters of Co-LOX protein showed the relative molecular mass was 91.65 kDa,the pI was 5.255,and a transmembrane domain was predicted in the second structure of the protein constructed mainly by a-helices.The distribution analysis results indicate that the protein appeared in chloroplast and cytoplasm.The phylogenetic investigation displays that Co-LOX shared homology with those of other species reaching up to 99%,such as C.sinensis,Vitis vinifera,Capsicum oleoresin,and Solannum tuberosum,and the relationship of Co-LOX and Olea europaea,Nicotiana tabacum,Lycopersicon esculentum and Actinidia chinensis reached up to 98%.