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2008•Qilu Pharmaceutical AffairsRequires access

Determination of camphor in Compound Benzoic Tincture by HPLC

YU Wei-bing

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Abstract

OBJECTIVE An HPLC method was developed to determine the camphor in Compound Benzoic Tincture.METHODS It was determined on a XDB C18 column,using methanol-water(80∶20)as mobile phase at a flow rate of 1.0 mL·min-1,and detection wavelength was 288nm.The column temperature was 25℃.RESULTS The linear relationship of the peak area was 0.4～2mg·mL-1by HPLC,with a regression coefficient of 0.9995.Average recovery was 100.07%,RSD=1.0%.CONCLUSION The method was simple,rapid and accurate.It can be used for the determination of camphor in Compound Benzoic Tincture.

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What this paper is about

OBJECTIVE An HPLC method was developed to determine the camphor in Compound Benzoic Tincture.METHODS It was determined on a XDB C18 column,using methanol-water(80∶20)as mobile phase at a flow rate of 1.0 mL·min-1,and detection wavelength was 288nm.The column temperature was 25℃.RESULTS The linear relationship of the peak area was 0.4～2mg·mL-1by HPLC,with a regression coefficient of 0.9995.Average recovery was 100.07%,RSD=1.0%.CONCLUSION The method was simple,rapid and accurate.It can be used for the determination of camphor in Compound Benzoic Tincture.

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Available abstract

OBJECTIVE An HPLC method was developed to determine the camphor in Compound Benzoic Tincture.METHODS It was determined on a XDB C18 column,using methanol-water(80∶20)as mobile phase at a flow rate of 1.0 mL·min-1,and detection wavelength was 288nm.The column temperature was 25℃.RESULTS The linear relationship of the peak area was 0.4～2mg·mL-1by HPLC,with a regression coefficient of 0.9995.Average recovery was 100.07%,RSD=1.0%.CONCLUSION The method was simple,rapid and accurate.It can be used for the determination of camphor in Compound Benzoic Tincture.

Key concepts: Tincture (heraldry), Camphor, Chromatography, Chemistry, Benzoic acid, High-performance liquid chromatography, Linear relationship, Organic chemistry

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