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2013•Shiyong yaowu yu linchuangRequires access

Determination of phenol in compound camphor liniment by HPLC

Guo Sheng-cai

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Abstract

Objective To establish an HPLC method for the determination of phenol in compound camphor liniment.Methods HPLC method was used.The column was ZORBAX Eclips XDB-C18(4.6 mm×200 mm,5 μm).The mobile phase consisted of methanol-water(70∶ 30)at a flow rate of 0.8 mL/min.The detector wavelength was set at 270 nm.Results The calibration curve of phenol was in good linearity over the range of 4.51~112.8 μg/mL,and the average recovery rate was 99.93%.Conclusion This method is rapid,accurate and reliable.It can be used in quality control and determination of phenol in compound camphor liniment.

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What this paper is about

Objective To establish an HPLC method for the determination of phenol in compound camphor liniment.Methods HPLC method was used.The column was ZORBAX Eclips XDB-C18(4.6 mm×200 mm,5 μm).The mobile phase consisted of methanol-water(70∶ 30)at a flow rate of 0.8 mL/min.The detector wavelength was set at 270 nm.Results The calibration curve of phenol was in good linearity over the range of 4.51~112.8 μg/mL,and the average recovery rate was 99.93%.Conclusion This method is rapid,accurate and reliable.It can be used in quality control and determination of phenol in compound camphor liniment.

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Available abstract

Objective To establish an HPLC method for the determination of phenol in compound camphor liniment.Methods HPLC method was used.The column was ZORBAX Eclips XDB-C18(4.6 mm×200 mm,5 μm).The mobile phase consisted of methanol-water(70∶ 30)at a flow rate of 0.8 mL/min.The detector wavelength was set at 270 nm.Results The calibration curve of phenol was in good linearity over the range of 4.51~112.8 μg/mL,and the average recovery rate was 99.93%.Conclusion This method is rapid,accurate and reliable.It can be used in quality control and determination of phenol in compound camphor liniment.

Key concepts: Camphor, Chromatography, High-performance liquid chromatography, Phenol, Chemistry, Calibration curve, Methanol, Detection limit

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