In Vitro Culture and Plant Regeneration from Cotyledon Explants of Capsicum chinense Jacq.

Effects of hormones on differentiation of adventitious buds and plant regeneration were studied in cotyledon culture of Capsicum chinense Jacquin in vitro. The best growth regulator for bud induction was found to be 6-BA. The combination of IAA and 6-BA raised the differentiation frequency obviously. GA3 added onto the differentiation medium promoted the elongation of adventitious buds. The differentiation medium MS + 6-BA 5 mg/L + IAA 1 mg/L + AgNO3 6 mg/L for adventitious buds was optimum and produced an average shoot bud inducing rate of more than 75%; the optimum shoot elongation medium for the adventitious buds was MS + 6-BA 3 mg/L + IAA 1 mg/L + AgNO3 6 mg/L + GA3 4 mg/L and gave an average elongation rate of 90%; the optimum rooting medium was MS + IAA 0.5 mg/L and produced an average rooting rate of 95%. A plant regeneration system for cotyledon explants of pepper was thus established.