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2007•Journal of the South China Agricultural UniversityRequires access

Plant Regeneration of Capsicum annuum Cultured in vitro

WU Zhi-ming

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Abstract

Effects of genotype,concerntration of plant growth regulator,AgNO_3 and CM on adventitious bud induction of pepper were studied using cotyledon with petiole as explants.The results showed that the best differentiation medium for adventitious buds was MB(MS_(mineral)+B_(5 Vitamin))+6-BA 5.0 mg/L+IAA 1.0 mg/L and its differentiation rate was 90.2%.The best elongation medium was MB+6-BA(3.0 mg/L)+IAA 1.0 mg/L+GA_3 2.0 mg/L+AgNO_3 10.0 mg/L+CM 10.0%(φ) and its highest elongation rate was 40.0%.Rooting medium was MS+IAA 0.2 mg/L+NAA 0.1 mg/L,and its average rooting rate was 85%.All the plantlets flowered and set fruits normally in the field.

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What this paper is about

Effects of genotype,concerntration of plant growth regulator,AgNO_3 and CM on adventitious bud induction of pepper were studied using cotyledon with petiole as explants.The results showed that the best differentiation medium for adventitious buds was MB(MS_(mineral)+B_(5 Vitamin))+6-BA 5.0 mg/L+IAA 1.0 mg/L and its differentiation rate was 90.2%.The best elongation medium was MB+6-BA(3.0 mg/L)+IAA 1.0 mg/L+GA_3 2.0 mg/L+AgNO_3 10.0 mg/L+CM 10.0%(φ) and its highest elongation rate was 40.0%.Rooting medium was MS+IAA 0.2 mg/L+NAA 0.1 mg/L,and its average rooting rate was 85%.All the plantlets flowered and set fruits normally in the field.

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Available abstract

Effects of genotype,concerntration of plant growth regulator,AgNO_3 and CM on adventitious bud induction of pepper were studied using cotyledon with petiole as explants.The results showed that the best differentiation medium for adventitious buds was MB(MS_(mineral)+B_(5 Vitamin))+6-BA 5.0 mg/L+IAA 1.0 mg/L and its differentiation rate was 90.2%.The best elongation medium was MB+6-BA(3.0 mg/L)+IAA 1.0 mg/L+GA_3 2.0 mg/L+AgNO_3 10.0 mg/L+CM 10.0%(φ) and its highest elongation rate was 40.0%.Rooting medium was MS+IAA 0.2 mg/L+NAA 0.1 mg/L,and its average rooting rate was 85%.All the plantlets flowered and set fruits normally in the field.

Key concepts: Petiole (insect anatomy), Cotyledon, Elongation, Biology, Explant culture, Horticulture, Murashige and Skoog medium, Pepper

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