Adventitious bud induction of S. integrifolium cotyledon and plantlet regeneration

【Objective】The present experiment was performed to study the effects of different hormone concentrations and combinations on induction of adventitious bud and root with the aim of establishing a highly efficient regeneration system of S.integrifolium to provide references for the research of eggplant genetic transformation. 【Method】With the cotyledon of S. integrifolium as explants, the different hormone combinations (0.5-2.0 mg/L 6-BA, 0.1-0.5 mg/L IAA and 0-0.5 mg/L IAA) were added in MS medium to induce the adventitious bud and root, respectively. Subsequently the optimal differential medium and rooting medium were screened. 【Result】The germination rate of adventitious buds increased with the increase in 6-BA concentration from 0.5 mg/L to 2.0 mg/L in MS medium. The germination rate of adventitious buds increased initially with IAA concentration increasing from 0.1 mg/L to 0.5 mg/L in MS medium, followed by a decrease. However, 2.0 mg/L 6-BA plus 0.3 mg/L IAA showed ideal induction effects on adventitious buds with 86% germination rate. As the IAA concentration in MS medium increased, the rooting rate decreased at first, then increased. The highest germination rate reached 100%. 【Conclusion】The best medium for initiation of adventitious bud of cotyledon was MS+2.0 mg/L 6-BA + 0.3 mg/L IAA, and the optimal rooting medium was MS+0.1 mg/L IAA.