Adventitious Buds Induction and Rapid Propagation from Leaflet Explants of Arachis hypogaea

The leaflet were used as explants to study plant regeneration,and the results showed that the adventitious bud differentiation could be effectively induced from the explants on medium MS +0.6mg/L NAA+8mg/L 6-BA+1mg/L AgNO3 + 3mg/L Gln,and shoot induction rate and average number of shoots per explant was 81.03 % and 5.79 respectively.The optimum medium for adventitions bud elongation was MS + 2mg/L 6-BA + 4mg/L GA3,and the average elongation length was 1.0-2.0 cm.The rooting rate of explants on 1/2 MS + 0.5mg/L NAA + 3mg/L IBA was 86.15 %,and the root was stronger,longer and had more lateral roots.After hardening-off,the survival rate and maturing rate reach to 90% and 100% respectively.