Establishment of highly efficient regeneration system with different explants of Sapium sebiserum in vitro.

A highly efficient regeneration system was established by culturing the different explants including embryos,endosperms with embryos,leaf and stem segments of Sapium sebiserum.The results showed that endosperm with embryos could be induced to form callus,and the frequency of callus induction was 90%.Many adventitious buds appeared on the callus culturing medium of MS+1.0 mg·L-1 NAA+1.0 mg·L-1 6-BA,and the number of adventitious buds was up to 15 from one callus.The percent of planting and germination of embryos was 100% in MS medium without plant regulator,but hypocotyls of embryo could be induced adventitious buds in MS medium including different content of 6-BA and NAA.The best medium for hypocotyls was MS+0.05 mg·L-1 NAA+0.3 mg·L-1 6-BA and the number of adventitious buds was up to 6.The optimal culture media for leaf segments and stem segments were MS+0.5 mg·L-1 NAA+0.5 mg·L-1 6-BA and MS+0.05 mg·L-1 NAA+0.1~0.3 mg·L-1 6-BA,the effective number of adventitious buds was 18 and 17,and the frequency of adventitious buds induction was 90% and 100%,respectively.The rooting rate of shoots was 100% in medium of 1/2MS+0.5 mg·L-1 IBA,but the roots of plantlet were thin plantlet were thin.The rooting rate of shoots was also 100% in medium of MS+1.0 mg·L-1 La3+,but the roots of plantlet were strong.The survival rate of transplants was 89.2% in greenhouse,and it was 68.9% in the field trial.