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2007•Lishizhen Medicine and Materia Medica ResearchRequires access

Content assay of Paeoniflorin in Qingxinpinggan Oral Liquid by HPLC

Yin Shou-yu

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Abstract

Objective To establish a HPLC method for content determination of paeoniflorin in Qingxinpinggan Oral Liquid.Methods HPLC were carried out using an Diamonsi C18(250 mm×4.6 mm,5 μm) column and mobile phase of Acetonitrile-0.1% phosphate(17∶83) under the 230 nm wavelength.Results The paeoniflorin showed a good linear correlation ship in the range of 0.18~3.6 μg(r=0.999 6).The average recovery ratio was 98.80%,RSD=0.36%(n=6).Conclusion The present method is simple,rapid and repeatable.It can be used for quality control of Qingxinpinggan Oral Liquid.

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What this paper is about

Objective To establish a HPLC method for content determination of paeoniflorin in Qingxinpinggan Oral Liquid.Methods HPLC were carried out using an Diamonsi C18(250 mm×4.6 mm,5 μm) column and mobile phase of Acetonitrile-0.1% phosphate(17∶83) under the 230 nm wavelength.Results The paeoniflorin showed a good linear correlation ship in the range of 0.18~3.6 μg(r=0.999 6).The average recovery ratio was 98.80%,RSD=0.36%(n=6).Conclusion The present method is simple,rapid and repeatable.It can be used for quality control of Qingxinpinggan Oral Liquid.

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Available abstract

Objective To establish a HPLC method for content determination of paeoniflorin in Qingxinpinggan Oral Liquid.Methods HPLC were carried out using an Diamonsi C18(250 mm×4.6 mm,5 μm) column and mobile phase of Acetonitrile-0.1% phosphate(17∶83) under the 230 nm wavelength.Results The paeoniflorin showed a good linear correlation ship in the range of 0.18~3.6 μg(r=0.999 6).The average recovery ratio was 98.80%,RSD=0.36%(n=6).Conclusion The present method is simple,rapid and repeatable.It can be used for quality control of Qingxinpinggan Oral Liquid.

Key concepts: Paeoniflorin, Chromatography, High-performance liquid chromatography, Chemistry, Content determination, Linear range, Detection limit

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