2016•Pharmacy TodayRequires access
Determination of Paeoniflorin in Qixuening Oral Liquid by HPLC
Lin Xiaochun, Weng Li-dong, Jinkun Zheng, LI Xugui, Huang Shengchan, Qiang Liu
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Abstract
OBJECTIVE METHODS RESULTS CONCLUSION To establish a method determination of Paeoniflorin in Qixuening oral liquid by HPLC. Methods: The Agilent HC-C18( 4.6 mm×250 mm,5 μm) was used as the column,acetonitrile-0. 2% phosphoric acid solution( 10 ∶90) was used as the mobile phase,the low rate was 1.0 m L / min and detected at a wavelength of230 nm,and the temperature was 24 ℃. Results: The content of Paeoniflorin was linear in the range of 0. 05- 0. 25 μg,the average recovery was 99.0%( r = 0.999 9) and RSD was 0.94%. The average results of Paeoniflorin content in the 12 batches of samples were0. 048 3 mg / m L. Conclusion: This method is simple,quick and accurate,for the determination of Paeoniflorin in Qixuening oral liquid.