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Determination of Paeoniflorin in Liqihewei Oral Liquid by HPLC

Zhu Yu-feng

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Abstract

Objective: To establish the quality control standard of Liqihewei Oral Liquid. Methods: Using HPLC with Hypersil C 18 columm (4.6 mm×250 mm,5 μm) The mobile phase was acetonitrile: 0.1% phosphoric acid solution (15∶85) with 1 ml/min of flow rate, and the detection wavelength was at 230 nm. Results: Paeoniflorin showed good linear relationship at the range of 0.153 5 μg～1.029 μg. The recovery was 98.892% and RSD was 2.69%. Conclusion: This method is easy, accurate and can be used for content determination of syring in Liqihewei Oral Liquid.

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What this paper is about

Objective: To establish the quality control standard of Liqihewei Oral Liquid. Methods: Using HPLC with Hypersil C 18 columm (4.6 mm×250 mm,5 μm) The mobile phase was acetonitrile: 0.1% phosphoric acid solution (15∶85) with 1 ml/min of flow rate, and the detection wavelength was at 230 nm. Results: Paeoniflorin showed good linear relationship at the range of 0.153 5 μg～1.029 μg. The recovery was 98.892% and RSD was 2.69%. Conclusion: This method is easy, accurate and can be used for content determination of syring in Liqihewei Oral Liquid.

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Available abstract

Objective: To establish the quality control standard of Liqihewei Oral Liquid. Methods: Using HPLC with Hypersil C 18 columm (4.6 mm×250 mm,5 μm) The mobile phase was acetonitrile: 0.1% phosphoric acid solution (15∶85) with 1 ml/min of flow rate, and the detection wavelength was at 230 nm. Results: Paeoniflorin showed good linear relationship at the range of 0.153 5 μg～1.029 μg. The recovery was 98.892% and RSD was 2.69%. Conclusion: This method is easy, accurate and can be used for content determination of syring in Liqihewei Oral Liquid.

Key concepts: Paeoniflorin, Chromatography, Phosphoric acid, High-performance liquid chromatography, Chemistry, Linear relationship, Linear range, Acetonitrile

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